1VPY
CRYSTAL STRUCTURE OF a DUF72 family protein (EF0366) FROM ENTEROCOCCUS FAECALIS V583 AT 2.52 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-27 |
| Detector | ADSC |
| Wavelength(s) | 0.979694, 0.979571, 1.019859 |
| Spacegroup name | P 65 |
| Unit cell lengths | 114.079, 114.079, 52.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.520 - 2.520 |
| R-factor | 0.22652 |
| Rwork | 0.225 |
| R-free | 0.25697 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.306 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4 4.2) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.520 | 2.660 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Number of reflections | 13332 | |
| <I/σ(I)> | 9.1 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 4.5 | 277 | 10.0% PEG-3000, 0.2M Zn(OAc)2, 0.1M Acetate, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K, pH 4.5 |
