1VPV
Crystal structure of a degv lipid binding protein (tm1468) from thermotoga maritima at 2.45 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-03 |
Detector | APS |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 103.256, 103.256, 115.863 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.160 - 2.450 |
R-factor | 0.18355 |
Rwork | 0.180 |
R-free | 0.25188 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mgp |
RMSD bond length | 0.017 |
RMSD bond angle | 1.549 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.453 | 2.453 |
Number of reflections | 22755 | |
<I/σ(I)> | 13.77 | 4.88 |
Completeness [%] | 96.4 | 97.78 |
Redundancy | 4.09 | 3.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.5 | 293 | 0.1M Cacodylate pH 6.5, 0.2M Mg(oAc)2, 27.5% PEG-8000, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |