1VPS
POLYOMAVIRUS VP1 PENTAMER COMPLEXED WITH A DISIALYLATED HEXASACCHARIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | FUJI |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 221.500, 221.500, 99.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNCOMPLEXED VP1 PENTAMER (PDB ENTRY 1VPN) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 * | |
| Number of reflections | 188556 | |
| <I/σ(I)> | 14.5 | 2.1 |
| Completeness [%] | 85.6 | 56.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | DROP: 1.0 M AMMONIUM PHOSPHATE PH 8.0 2.5 & ETHANOL 8-10 MG/ML PROTEIN RESERVOIR: 2.0 M AMMONIUM PHOSPHATE PH 8.0 5 % ETHANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium phosphate | 1.0 (M) | |
| 2 | 1 | drop | ethanol | 2.5 (%) | |
| 3 | 1 | drop | protein | 8-10 (mg/ml) | |
| 4 | 1 | reservoir | ammonium phosphate | 2.0 (M) | |
| 5 | 1 | reservoir | ethanol | 5 (%) |
