1VPR

Crystal structure of a luciferase domain from the dinoflagellate Lingulodinium polyedrum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979070, 0.979259, 0.994904
Spacegroup name	P 21 21 21
Unit cell lengths	58.989, 63.955, 96.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.600 - 1.800
R-factor	0.199
Rwork	0.199
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	1.900
High resolution limit [Å]	1.700	1.800
Rmerge	0.035
Number of reflections	35921
<I/σ(I)>	20.4	3
Completeness [%]	99.0	99
Redundancy	4	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	287	MEPEG 2000, 100mM EPPS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K