1VPM
Crystal structure of Acyl-CoA hydrolase (NP_241664.1) from Bacillus halodurans at 1.66 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-09 |
Detector | ADSC |
Wavelength(s) | 0.979764,1.019943,0.979648 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 82.984, 106.507, 120.254 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 1.660 |
R-factor | 0.17146 |
Rwork | 0.170 |
R-free | 0.19669 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.728 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.750 | 1.750 |
High resolution limit [Å] | 1.660 | 1.660 |
Number of reflections | 62701 | |
<I/σ(I)> | 13 | 2.8 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 5.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 0.15M Na formate, 20.00% PEG 3350, 0.033M Cl2E9, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 0.15M Na formate, 20.00% PEG 3350, 0.033M Cl2E9, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |