1VPL
Crystal structure of ABC transporter ATP-binding protein (TM0544) from Thermotoga maritima at 2.10 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.590, 90.590, 72.238 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.230 - 2.100 |
R-factor | 0.17372 |
Rwork | 0.171 |
R-free | 0.21581 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ji0 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.667 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.230 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 20216 | |
<I/σ(I)> | 13.6 | 2 |
Completeness [%] | 99.2 | 92.1 |
Redundancy | 8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 4 | 277 | 0.8M (NH4)2SO4, 0.1M Citrate pH 4.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |