1VPI

PHOSPHOLIPASE A2 INHIBITOR FROM VIPOXIN

Experimental procedure

Source type	SEALED TUBE
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1993-06
Detector	OIIF, DUBNA, RUSSIA
Spacegroup name	P 43 21 2
Unit cell lengths	68.900, 68.900, 52.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.760
R-factor	0.155
Rwork	0.155
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pp2
RMSD bond length	0.012
RMSD bond angle	22.250 *
Data reduction software	BLANC
Data scaling software	BLANC
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.860
High resolution limit [Å]	1.760	1.760
Rmerge	0.062
Total number of observations	42158 *
Number of reflections	11854
Completeness [%]	98.0	48
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3		PROTEIN WAS CRYSTALLIZED FROM 52% AMMONIUM SULFATE, 0.5% MPD, 100 MM TRIS, PH 8.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	ammonium sulfate	52 (%sat)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	MPD	0.5 (%(v/v))