1VPE
CRYSTALLOGRAPHIC ANALYSIS OF PHOSPHOGLYCERATE KINASE FROM THE HYPERTHERMOPHILIC BACTERIUM THERMOTOGA MARITIMA
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.009, 76.864, 87.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.28800 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM (V. 5.23) |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | |
| Total number of observations | 106055 * | |
| Number of reflections | 27144 | |
| Completeness [%] | 93.2 | 74.3 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 20 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | Mops | 5 (mM) | |
| 3 | 1 | drop | AMP-PNP | 2 (mM) | |
| 4 | 1 | drop | 3-phosphoglycerate | 2 (mM) | |
| 5 | 1 | drop | 2 (mM) | ||
| 6 | 1 | reservoir | PEG3000 | 5 (%) | |
| 7 | 1 | reservoir | PEG8000 | 20 (%) |
