1VP8
CRYSTAL STRUCTURE OF an alpha/beta domain of a putative pyruvate kinase (AF0103) FROM ARCHAEOGLOBUS FULGIDUS DSM 4304 AT 1.30 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-28 |
Detector | ADSC |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 109.650, 109.650, 76.451 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.610 - 1.300 |
R-factor | 0.15499 |
Rwork | 0.154 |
R-free | 0.16633 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.620 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.900 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 66691 | |
<I/σ(I)> | 21.9 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.4 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 50.0% PEG-200, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 50.0% PEG-200, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 50.0% PEG-200, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |