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1VP7

Crystal structure of Exodeoxyribonuclease VII small subunit (NP_881400.1) from Bordetella pertussis at 2.40 A resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]100
Detector technologyCCD
Collection date2004-09-18
DetectorADSC
Spacegroup nameP 6 2 2
Unit cell lengths107.030, 107.030, 207.260
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution29.020 - 2.400
R-factor0.20473
Rwork0.203
R-free0.24440
Structure solution methodMAD
RMSD bond length0.016
RMSD bond angle1.605
Data reduction softwareMOSFLM
Data scaling softwareSCALA (5.0))
Phasing softwareautoSHARP
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]29.0202.530
High resolution limit [Å]2.4002.400
Number of reflections28264
<I/σ(I)>183.6
Completeness [%]99.9100
Redundancy11.76.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION,SITTING DROP,NANODROP7.52772.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1VAPOR DIFFUSION,SITTING DROP,NANODROP7.52772.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1VAPOR DIFFUSION,SITTING DROP,NANODROP7.52772.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K

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