1VP7
Crystal structure of Exodeoxyribonuclease VII small subunit (NP_881400.1) from Bordetella pertussis at 2.40 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-18 |
Detector | ADSC |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 107.030, 107.030, 207.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.020 - 2.400 |
R-factor | 0.20473 |
Rwork | 0.203 |
R-free | 0.24440 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.605 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (5.0)) |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.020 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 28264 | |
<I/σ(I)> | 18 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |