1VNC

CHLOROPEROXIDASE FROM THE FUNGUS CURVULARIA INAEQUALIS

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	131.690, 131.690, 112.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.2
Rwork	0.200
RMSD bond length	0.012
RMSD bond angle	22.900 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.100	2.100 *
Rmerge	0.094 *
Total number of observations	132089 *
Number of reflections	42197 *
Completeness [%]	98.9	96.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.5 (mg/ml)
2	1	drop	Mops	3.75 (mM)
3	1	drop		0.75 (M)
4	1	drop	ammonium sulfate	0.375 (M)
5	1	drop	Tris-HCl	0.025 (M)
6	1	drop		0.005 (%)
7	1	reservoir	ammonium sulfate	1.6 (M)
8	1	reservoir	Tris-HCl	0.1 (M)
9	1	reservoir		0.02 (%)