1VMG
Crystal structure of MazG nucleotide pyrophosphohydrolase (13816655) from Sulfolobus solfataricus at 1.46 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-18 |
| Detector | ADSC |
| Wavelength(s) | 0.979834,0.979694,1.020035 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 79.749, 79.749, 95.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.620 - 1.460 |
| R-factor | 0.14201 |
| Rwork | 0.142 |
| R-free | 0.14633 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.235 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC ((5.2.0005)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.620 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 25360 | |
| <I/σ(I)> | 19.6 | 2.2 |
| Completeness [%] | 93.3 | 57.6 |
| Redundancy | 5.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 1.5M Li2SO4, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
