1VME

Crystal structure of Flavoprotein (TM0755) from Thermotoga maritima at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.898407, 0.979029
Spacegroup name	P 1 21 1
Unit cell lengths	55.242, 95.831, 90.130
Unit cell angles	90.00, 95.43, 90.00

Refinement procedure

Resolution	48.920 - 1.800
R-factor	0.14858
Rwork	0.147
R-free	0.18104
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.553
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC ((5.2.0005))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	85995
<I/σ(I)>	22.6	3
Completeness [%]	100.0	99.9
Redundancy	12.2	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	35.0% MPD, 0.1M Acetate pH 4.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	35.0% MPD, 0.1M Acetate pH 4.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	35.0% MPD, 0.1M Acetate pH 4.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP