1VMA
Crystal structure of Cell division protein ftsY (TM0570) from Thermotoga maritima at 1.60 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-02 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.446, 48.102, 90.792 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 24.880 - 1.600 |
| R-factor | 0.20905 |
| Rwork | 0.207 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fts |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA ((CCP4 4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC ((5.2.0001)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.880 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 84144 | |
| <I/σ(I)> | 18.2 | 3.2 |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 8.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 277 | 10.0% PEG-6000, 0.1M Citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
