1VM6
Crystal structure of Dihydrodipicolinate reductase (TM1520) from Thermotoga maritima at 2.27 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.077, 109.217, 112.602 |
| Unit cell angles | 90.00, 119.23, 90.00 |
Refinement procedure
| Resolution | 29.030 - 2.270 |
| R-factor | 0.17334 |
| Rwork | 0.171 |
| R-free | 0.21256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1arz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.596 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.030 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 61963 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 47.8 | 38.4 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 4.5 | 293 | 20.0% PEG-400, 0.2M Ca(OAc)2, 0.1M Acetate pH 4.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
