1VM0

X-RAY STRUCTURE OF GENE PRODUCT FROM ARABIDOPSIS THALIANA AT2G34160

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.97908,0.97931,0.97623
Spacegroup name	P 21 21 2
Unit cell lengths	59.785, 79.079, 43.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.361 - 1.800
R-factor	0.17675
Rwork	0.174
R-free	0.22060
RMSD bond length	0.023
RMSD bond angle	2.001
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.068	0.035	0.329
Total number of observations		3748	3544
Number of reflections	37012
<I/σ(I)>	14	3.7	3.7
Completeness [%]	99.4	99.9	95.7
Redundancy	4.5	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	7	293	protein 10 mg/ml, 18% PEG2K, potassium nitrate 0.200 M, MOPS 0.100 M, pH 7.0, Batch, temperature 293 K