1VLY

Crystal structure of a putative aminomethyltransferase (ygfz) from escherichia coli at 1.30 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 1
Unit cell lengths	44.814, 50.080, 52.014
Unit cell angles	94.68, 115.29, 116.46

Refinement procedure

Resolution	26.450 - 1.300
R-factor	0.13572
Rwork	0.134
R-free	0.16785
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.495
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.450	1.370
High resolution limit [Å]	1.300	1.300
Number of reflections	75749
<I/σ(I)>	17.5	3.2
Completeness [%]	89.3	61.5
Redundancy	3.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.3	277	0.2M CaAcetate, 20.0% PEG-3350, No Buffer pH 7.3, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.3	277	0.2M CaAcetate, 20.0% PEG-3350, No Buffer pH 7.3, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K