1VLV
Crystal structure of Ornithine carbamoyltransferase (TM1097) from Thermotoga maritima at 2.25 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-20 |
Detector | ADSC |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 210.339, 210.339, 210.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.320 - 2.250 |
R-factor | 0.19049 |
Rwork | 0.189 |
R-free | 0.21528 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a1s |
RMSD bond length | 0.015 |
RMSD bond angle | 1.576 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.320 | 2.310 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 19349 | |
<I/σ(I)> | 15.7 | 5014 |
Completeness [%] | 99.8 | 98.1 |
Redundancy | 6.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.1 | 293 | 0.2% MPD, 0.2M NH4OAc, 0.1M Na,K-Phosphate pH 6.1, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |