1VLU

Crystal structure of Gamma-glutamyl phosphate reductase (yor323c) from Saccharomyces cerevisiae at 2.40 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-02
Detector	ADSC QUANTUM 315
Spacegroup name	C 2 2 21
Unit cell lengths	123.041, 191.081, 125.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.830 - 2.290
R-factor	0.2123
Rwork	0.210
R-free	0.24782
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.238
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	2.360
High resolution limit [Å]	2.290	2.290
Rmerge	0.100	0.513
Number of reflections	60572
<I/σ(I)>	13	1.2
Completeness [%]	91.7	51.7
Redundancy	4.5	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.175M Na Cl, 0.06M Acetic Acid, 20.00% MPD, 0.04M Acetate_Na, 0.01M Cymal , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.175M Na Cl, 0.06M Acetic Acid, 20.00% MPD, 0.04M Acetate_Na, 0.01M Cymal , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.175M Na Cl, 0.06M Acetic Acid, 20.00% MPD, 0.04M Acetate_Na, 0.01M Cymal , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K