1VLS

LIGAND BINDING DOMAIN OF THE WILD-TYPE ASPARTATE RECEPTOR

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	I 41
Unit cell lengths	65.020, 65.020, 72.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.850
R-factor	0.197
Rwork	0.197
RMSD bond length	0.010 *
RMSD bond angle	1.600 *
Data reduction software	MSC
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.850 *
Rmerge	0.072
Total number of observations	51445 *
Number of reflections	12189 *
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.1 (M)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	Na acetate	1.0 (M)
4	1	drop		0.05 (M)
5	1	drop	HEPES	0.5 (M)
6	1	drop	Na acetate	0.50 (M)
7	1	drop	protein	10 (mg/ml)