1VLO

Crystal structure of aminomethyltransferase (T protein; tetrahydrofolate-dependent) of glycine cleavage system (np417381) from Escherichia coli k12 at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	46.517, 64.564, 117.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.380 - 1.700
R-factor	0.16051
Rwork	0.159
R-free	0.19514
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.345
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.380	1.740
High resolution limit [Å]	1.700	1.700
Number of reflections	38018
<I/σ(I)>	11.1	2.5
Completeness [%]	96.2	81.5
Redundancy	3.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	20.0% PEG-10000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	20.0% PEG-10000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	20.0% PEG-10000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	20.0% PEG-10000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K