1VLN

A TRICLINIC CRYSTAL FORM OF THE LECTIN CONCANAVALIN A

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-12-25
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	78.800, 79.300, 133.300
Unit cell angles	97.10, 90.20, 97.50

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.205
Rwork	0.205
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vam
RMSD bond length	0.010
RMSD bond angle	26.900 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.174	0.799 *
Number of reflections	116878
Completeness [%]	94.1	94 *
Redundancy	3.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8 *	4 *	buffer was added in a 1:1 ratio to the protein solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	70 (mg/ml)
2	1	1	Tris-HCl	20 (mM)
3	1	1		20 (mM)
4	1	1		20 (mM)
5	1	1	Tris-HCl	10 (mM)	buffer
6	1	1	ammonium sulfate	1 (M)