1VLM
Crystal structure of SAM-dependent methyltransferase, possible histamine N-methyltransferase (TM1293) from Thermotoga maritima at 2.20 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-12 |
Detector | ADSC |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.648, 70.734, 93.922 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.810 - 2.200 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.24610 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.445 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.810 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 22405 | |
<I/σ(I)> | 9.9 | 2 |
Completeness [%] | 98.4 | 92.1 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 20.00% NP_Isopropanol, 0.034M Na/K-Phosphate pH5.0, 0.066M Na/K-Phosphate pH 7.0, 5.00% Glycerol, 20.00% NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 20.00% NP_Isopropanol, 0.034M Na/K-Phosphate pH5.0, 0.066M Na/K-Phosphate pH 7.0, 5.00% Glycerol, 20.00% NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 20.00% NP_Isopropanol, 0.034M Na/K-Phosphate pH5.0, 0.066M Na/K-Phosphate pH 7.0, 5.00% Glycerol, 20.00% NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |