1VLH
Crystal structure of Phosphopantetheine adenylyltransferase (TM0741) from Thermotoga maritima at 2.20 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-08 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.963, 110.876, 74.087 |
| Unit cell angles | 90.00, 111.98, 90.00 |
Refinement procedure
| Resolution | 68.700 - 2.200 |
| R-factor | 0.17332 |
| Rwork | 0.171 |
| R-free | 0.22687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b6t |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.716 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.700 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 48776 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 94.5 | 68 |
| Redundancy | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 8 | 277 | 0.2M MgCl2, 35.0% MPD, 0.1M Imidazole pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
