1VLC
Crystal structure of 3-isopropylmalate dehydrogenase (TM0556) from Thermotoga maritima at 1.90 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-27 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 118.780, 118.780, 56.699 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.010 - 1.900 |
R-factor | 0.18934 |
Rwork | 0.188 |
R-free | 0.21822 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.673 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.010 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.110 | 0.684 |
Number of reflections | 36394 | |
<I/σ(I)> | 13.6 | 1.9 |
Completeness [%] | 99.5 | 97.2 |
Redundancy | 5.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 9 | 277 | 25.0% PEG-4000, 0.2M Li2SO4, 0.1M TRIS pH 9.0 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 9 | 277 | 25.0% PEG-4000, 0.2M Li2SO4, 0.1M TRIS pH 9.0 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 9 | 277 | 25.0% PEG-4000, 0.2M Li2SO4, 0.1M TRIS pH 9.0 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |