1VLA
Crystal structure of Hydroperoxide resistance protein OsmC (TM0919) from Thermotoga maritima at 1.80 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-30 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.754, 39.789, 110.448 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 29.320 - 1.800 |
| R-factor | 0.17292 |
| Rwork | 0.170 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ml8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.517 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.320 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 51597 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 98.1 | 94.2 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 0.2M CaCl2, 20% PEG-3350 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
