1VKZ

Crystal structure of Phosphoribosylamine--glycine ligase (TM1250) from Thermotoga maritima at 2.30 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-03
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	63.745, 78.443, 85.018
Unit cell angles	90.00, 95.06, 90.00

Refinement procedure

Resolution	37.260 - 2.300
R-factor	0.21572
Rwork	0.213
R-free	0.26383
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.491
Data reduction software	DENZO
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.632
Number of reflections	31749
<I/σ(I)>	12.1	1.3
Completeness [%]	85.6	88.2
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	18% PEG-8000, 0.1M sodium cacodylate pH 6.5, 0.2M calcium acetate hydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	18% PEG-8000, 0.1M sodium cacodylate pH 6.5, 0.2M calcium acetate hydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	18% PEG-8000, 0.1M sodium cacodylate pH 6.5, 0.2M calcium acetate hydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K