1VKY

Crystal structure of S-adenosylmethionine tRNA ribosyltransferase (TM0574) from Thermotoga maritima at 2.00 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-18
Detector	ADSC
Spacegroup name	I 2 2 2
Unit cell lengths	119.033, 122.843, 131.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.390 - 2.000
R-factor	0.18107
Rwork	0.180
R-free	0.21041
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.529
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.390	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.073	0.678
Number of reflections	65036
<I/σ(I)>	14.8	2.7
Completeness [%]	100.0	100
Redundancy	7.3	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		295	0.10M NP_Citrate 1, 4.0% NP_Peg 6000, % NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 295K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		295	0.10M NP_Citrate 1, 4.0% NP_Peg 6000, % NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 295K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		295	0.10M NP_Citrate 1, 4.0% NP_Peg 6000, % NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 295K