1VKX
CRYSTAL STRUCTURE OF THE NFKB P50/P65 HETERODIMER COMPLEXED TO THE IMMUNOGLOBULIN KB DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 106.610, 106.610, 206.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMEN |
| Starting model (for MR) | ONE MONOMER OF P50/DNA COMPLEX AND ONE MONO OF P65/DNA COMPLEX |
| RMSD bond length | 0.014 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.082 | 0.450 |
| Total number of observations | 88449 * | |
| Number of reflections | 26430 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 97.0 | 88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 18 * | pH 5.50, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM ACETATE | ||
| 2 | 1 | 1 | CACL2 | ||
| 3 | 1 | 1 | BETA-OCTYL-GLUCOSIDE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | DITHIOTHREITOL | ||
| 6 | 1 | 1 | PEG 3350 | ||
| 7 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 8 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
