1VKM
Crystal structure of an indigoidine synthase a (idga)-like protein (tm1464) from thermotoga maritima msb8 at 1.90 A resolution
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-12 |
Detector | ADSC |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 91.958, 130.799, 138.750 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
分解能 | 75.230 - 1.900 |
R因子 | 0.15618 |
Rwork | 0.154 |
R-free | 0.19959 |
Structure solution method | MAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
結合角の平均二乗偏差(RMSD) [度] | 1.514 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0000) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 75.230 | 1.950 |
分解能 [Å] (高) | 1.900 | 1.900 |
Rmerge_l_obs | 0.063 | 0.533 |
独立反射数 | 130700 | |
<I/σ(I)> | 13.7 | 1.9 |
完全性 [%] | 99.1 | 93.3 |
冗長性 | 4.7 | 2.8 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.4 | 277 | 7% PEG 1000, 0.045M HEPES_Na, 0.055M HEPES, 0.01M MANGANESE (II) CHLORIDE, pH 7.4, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.4 | 277 | 7% PEG 1000, 0.045M HEPES_Na, 0.055M HEPES, 0.01M MANGANESE (II) CHLORIDE, pH 7.4, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.4 | 277 | 7% PEG 1000, 0.045M HEPES_Na, 0.055M HEPES, 0.01M MANGANESE (II) CHLORIDE, pH 7.4, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |