1VKK
Crystal structure of Glia maturation factor-gamma (GMFG) from Mus musculus at 1.50 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-08 |
| Detector | ADSC |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.049, 34.605, 38.365 |
| Unit cell angles | 69.95, 87.51, 70.46 |
Refinement procedure
| Resolution | 35.920 - 1.350 |
| R-factor | 0.15996 |
| Rwork | 0.158 |
| R-free | 0.19411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f7s |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.564 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.920 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 22189 | |
| <I/σ(I)> | 16.4 | 2.2 |
| Completeness [%] | 76.6 | 23.8 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 8.5 | 277 | 30% PEG-4000, 0.1M Tris hydrochloride pH 8.5,0.2M lithium sulfate monohydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
