1VKI
Crystal structure of a putative oligo-nucleotide binding protein (atu3699, agr_l_2275) from agrobacterium tumefaciens str. c58 at 1.60 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-01 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.997, 64.848, 77.427 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 49.340 - 1.600 |
| R-factor | 0.15366 |
| Rwork | 0.152 |
| R-free | 0.18496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.497 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 44165 | |
| <I/σ(I)> | 19.88 | 2.4 |
| Completeness [%] | 95.4 | 71.07 |
| Redundancy | 2.56 | 1.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 20.025% PEG 8000, 0.08M HEPES, 0.02M HEPES_Na , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
