1VKH
CRYSTAL STRUCTURE OF A PUTATIVE SERINE HYDROLASE (YDR428C) FROM SACCHAROMYCES CEREVISIAE AT 1.85 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-13 |
| Detector | ADSC |
| Wavelength(s) | 0.9464, 0.979326, 0.979179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.967, 85.959, 70.479 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 37.440 - 1.850 |
| R-factor | 0.14523 |
| Rwork | 0.143 |
| R-free | 0.18940 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.698 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 42225 | |
| <I/σ(I)> | 17.88 | 3.57 |
| Completeness [%] | 99.8 | 98.39 |
| Redundancy | 3.89 | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 298 | 16.00% PEG MME 2000, 0.10M HEPES, 0.00M HEPES_Na, 5% Glycerol , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 298K |
