1VKE
Crystal structure of carboxymuconolactone decarboxylase family protein possibly involved in antioxidative response (TM1620) from Thermotoga maritima at 1.56 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-24 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.922, 67.975, 90.077 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 44.650 - 1.560 |
| R-factor | 0.15918 |
| Rwork | 0.158 |
| R-free | 0.17834 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p8c |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.440 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 106734 | |
| <I/σ(I)> | 32.69 | 1.32 |
| Completeness [%] | 92.3 | 55.27 |
| Redundancy | 3.8 | 1.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 5.5 MES 25 MPD 0.2 Li2SO4, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
