1VKD
Crystal structure of a predicted glycosidase (tm1225) from thermotoga maritima msb8 at 2.10 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-04 |
Detector | ADSC |
Wavelength(s) | 1.115896, 0.979508, 0.979740, 1.019778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.867, 100.908, 253.433 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.170 - 2.100 |
R-factor | 0.16417 |
Rwork | 0.162 |
R-free | 0.20404 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.418 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.910 | 2.150 |
High resolution limit [Å] | 2.098 | 2.100 |
Number of reflections | 119326 | |
<I/σ(I)> | 11.3 | 1.5 |
Completeness [%] | 91.2 | 78.4 |
Redundancy | 4.1 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.5 | 293 | Cacodylate pH 6.5, 5% PEG-1000, 0.2M MgCl2 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |