1VKC
Putative acetyl transferase from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SBC-3 |
| Wavelength(s) | 0.97702 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.919, 67.387, 49.414 |
| Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.890 |
| R-factor | 0.21098 |
| Rwork | 0.207 |
| R-free | 0.24419 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.078 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.2.0001) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.890 | 4.090 | 1.890 |
| Rmerge | 0.049 | 0.025 | 0.442 |
| Number of reflections | 24184 | ||
| Completeness [%] | 99 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.9 | 295 | 8% (v/v) MPD, 100mM magnesium chloride, 100mM sodium citrate, pH 5.9, micro batch, temperature 295K |
