1VKA

Southeast Collaboratory for Structural Genomics: Hypothetical Human Protein Q15691 N-Terminal Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Detector technology	CCD
Collection date	2003-02-15
Detector	MAR CCD 165 mm
Spacegroup name	P 1 21 1
Unit cell lengths	31.951, 47.582, 100.690
Unit cell angles	90.00, 91.74, 90.00

Refinement procedure

Resolution	100.000 - 1.600
Rwork	0.200
R-free	0.21370
Structure solution method	sulfur sas
RMSD bond length	0.014
RMSD bond angle	1.185
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.040	0.033	0.107
Number of reflections	37287
<I/σ(I)>	26.2
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Micro-batch under oil	6.2	291	pH 6.2, Micro-batch under oil, temperature 291K
1	Micro-batch under oil	6.2	291	pH 6.2, Micro-batch under oil, temperature 291K