1VK9
CRYSTAL STRUCTURE OF A DUF1893 family protein (TM1506) FROM THERMOTOGA MARITIMA AT 2.70 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-12 |
| Detector | ADSC |
| Wavelength(s) | 0.9796, 1.0332, 0.9795 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 132.891, 132.891, 66.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.500 - 2.700 |
| R-factor | 0.20789 |
| Rwork | 0.206 |
| R-free | 0.25083 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.380 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 9700 | |
| <I/σ(I)> | 15.7 | 2 |
| Completeness [%] | 97.4 | 78 |
| Redundancy | 6.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5.8 | 277 | 40% PEG-600, 0.1M Imidazole pH 8.0, 0.2M Zn(OAc)2, pH 5.8, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
