1VK5

X-ray Structure of Gene Product from Arabidopsis Thaliana At3g22680

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	P 31 2 1
Unit cell lengths	83.450, 83.450, 60.575
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.300 - 1.604
R-factor	0.16102
Rwork	0.160
R-free	0.18350
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.646
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.05)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.037	0.021	0.302
Number of reflections	32231
<I/σ(I)>	38.61		9.3
Completeness [%]	99.2	99.8	92.2
Redundancy	10.9		10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	protein (10 mg/ml), Ammonium Sulfate (0.8M), Hepes (0.1M), Magnesium Sulfate (0.04M), CHAPS (0.41%), pH 8.5, Vapor diffusion, hanging drop, temperature 293K
2	VAPOR DIFFUSION, HANGING DROP	8.5	293	protein (5 mg/ml), Ammonium Sulfate (0.8M), Hepes (0.1M), Magnesium Sulfate (0.04M), CHAPS (0.41%), pH 8.5, Vapor diffusion, hanging drop, temperature 293K