1VK4
Crystal structure of PfkB Carbohydrate kinase (TM0415) from Thermotoga maritima at 1.91 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.939244, 0.979414, 0.979121 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.290, 68.530, 96.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.770 - 1.910 |
| R-factor | 0.17007 |
| Rwork | 0.168 |
| R-free | 0.21938 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 20420 | |
| <I/σ(I)> | 27.3 | 9.4 |
| Completeness [%] | 89.4 | 58 |
| Redundancy | 12.6 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7 | 277 | TRIS pH 7, 15% Ethanol , pH 7.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
