1VK3

Crystal structure of Phosphoribosylformylglycinamidine synthase II (TM1246) from Thermotoga maritima at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-12
Detector	ADSC
Wavelength(s)	1.0035, 0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	59.775, 72.689, 128.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.260 - 2.150
R-factor	0.18974
Rwork	0.186
R-free	0.25423
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.315
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.690	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	28272
<I/σ(I)>	10.2	1.3
Completeness [%]	90.8	56.8
Redundancy	3.2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8	293	30.00% PEG 200, 0.10M Tris pH 8.0, 7% NP_PEG 4000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K