1VK0

X-ray Structure of Gene Product from Arabidopsis Thaliana At5g06450

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-26
Detector	MARRESEARCH
Wavelength(s)	0.9792, 0.9800, 0.9641
Spacegroup name	P 32 2 1
Unit cell lengths	120.831, 120.831, 185.222
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.922 - 2.100
R-factor	0.1829
Rwork	0.180
R-free	0.23260
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	1.777
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.02)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.140
High resolution limit [Å]	2.100	5.690	2.100
Rmerge	0.062	0.031	0.479
Number of reflections	91738
<I/σ(I)>	29		5.8
Completeness [%]	100.0	100	99.8
Redundancy	10.6		8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Sodium Citrate, Polyethylene glycol 2000, hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K