1VJZ

Crystal structure of Endoglucanase (TM1752) from Thermotoga maritima at 2.05 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-20
Detector	APS-1
Spacegroup name	P 43 21 2
Unit cell lengths	64.684, 64.684, 202.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.340 - 2.050
R-factor	0.17021
Rwork	0.168
R-free	0.21244
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.433
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.095	0.334
Number of reflections	26538
<I/σ(I)>	14.57	3
Completeness [%]	94.6	75.23
Redundancy	3.82	2.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	9	277	Bicine pH 9, 2% Dioxane, 10% PEG-20000, pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	9	277	Bicine pH 9, 2% Dioxane, 10% PEG-20000, pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	9	277	Bicine pH 9, 2% Dioxane, 10% PEG-20000, pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K