1VJW

STRUCTURE OF OXIDOREDUCTASE (NADP+(A),FERREDOXIN(A))

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-08-08
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	29.630, 38.020, 44.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.750
R-factor	0.159
Rwork	0.159
RMSD bond length	0.009
RMSD bond angle	1.856
Data reduction software	MOSFLM (V5.23)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.200 *
High resolution limit [Å]	1.700 *	1.750 *
Rmerge	0.078
Total number of observations	36264 *
Number of reflections	5864
Completeness [%]	97.9	93.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	ammonium sulfate	3.2 (M)
3	1	drop	Tris-HCl	0.1 (M)
4	1	reservoir	ammonium sulfate	3.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)