1VJT
Crystal structure of Alpha-glucosidase (TM0752) from Thermotoga maritima at 2.50 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-08 |
| Detector | APS |
| Wavelength(s) | 1.00000, 0.979340, 0.979206 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.722, 79.815, 89.222 |
| Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
| Resolution | 43.640 - 2.500 |
| R-factor | 0.19754 |
| Rwork | 0.194 |
| R-free | 0.25602 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.171 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15162 | |
| <I/σ(I)> | 19.08 | 3.11 |
| Completeness [%] | 83.3 | 42.11 |
| Redundancy | 3.12 | 2.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 8.1 | 277 | 0.2M tri-lithium citrate tetrahydrate, 20% PEG-3350, pH 8.1, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
