1VJO

Crystal structure of Alanine--glyoxylate aminotransferase (ALR1004) from Nostoc sp. at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-29
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	79.258, 101.057, 97.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.180 - 1.700
R-factor	0.15383
Rwork	0.151
R-free	0.20200 *
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.590 *
Data reduction software	DENZO
Data scaling software	SCALA (4.2))
Phasing software	SHELXD
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.180	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.069 *	0.431 *
Total number of observations	313619 *
Number of reflections	39819 *
<I/σ(I)>	11.8	2
Completeness [%]	92.0 *	74.9 *
Redundancy	5.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8 *	293	0.06M HEPES, 0.04M HEPES_Na, 12% PEG MME 5000, 0.2M NP_Calcium Chloride , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	12 (%)
2	1	reservoir		0.2 (M)	pH7.5
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	ethylene glycol	10 (%)
5	1	drop	Tris	10 (mM)	pH7.8
6	1	drop		150 (mM)
7	1	drop	protein	22 (mg/ml)