1VJL

Crystal structure of a duf151 family protein (tm0160) from thermotoga maritima at 1.90 A resolution

Replaces:  1O5Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-15
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	43.509, 51.061, 72.974
Unit cell angles	90.00, 97.39, 90.00

Refinement procedure

Resolution	32.960 - 1.900
R-factor	0.201
Rwork	0.198
R-free	0.25300
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.665
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.086	0.485
Number of reflections	24182
<I/σ(I)>	19.58	1.44
Completeness [%]	100.0	92.4
Redundancy	2.18	1.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	293	10 % isopropanol, 20 % PEG 4000; 0.1 M HEPES pH7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	293	10 % isopropanol, 20 % PEG 4000; 0.1 M HEPES pH7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	293	10 % isopropanol, 20 % PEG 4000; 0.1 M HEPES pH7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K