1VJK
Putative molybdopterin converting factor, subunit 1 from Pyrococcus furiosus, Pfu-562899-001
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 81.467, 81.467, 63.698 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.711 - 1.510 |
Rwork | 0.210 |
R-free | 0.22430 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.322 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.560 |
High resolution limit [Å] | 1.510 | 3.250 | 1.510 |
Rmerge | 0.065 | 0.058 | 0.160 |
Number of reflections | 19993 | ||
Completeness [%] | 99.3 | 93.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | modified microbatch | 6.5 | 291 | 100mM sodium cacodylate, 30% PEG 8000, 200mM ammonium sulfate, modified microbatch, temperature 291K, pH 6.5 |