1VJI
Gene Product of At1g76680 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Detector technology | CCD |
Collection date | 2003-10-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.96400 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.688, 88.066, 149.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.850 - 2.000 * |
Rwork | 0.206 |
R-free | 0.29300 |
RMSD bond length | 0.024 |
RMSD bond angle | 1.890 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE (CCP4 4.2) |
Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.850 * | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 4.930 | 2.000 |
Rmerge | 0.065 * | 0.025 | 0.816 * |
Number of reflections | 20780 * | ||
Completeness [%] | 98.2 | 99.9 | 92 |
Redundancy | 3.6 * | 3.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | Methyl ether, Polyethylene glycol 5k,Calcium Chloride, MES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | TCEP | 0.3 (mM) | |
5 | 1 | reservoir | PEG5000 MME | 8.75-10 (%) | |
6 | 1 | reservoir | TEA | 0.1 (M) | pH8.0 |
7 | 1 | reservoir | glycine | 0.275-0.35 (M) |