1VJF
CRYSTAL STRUCTURE OF A PUTATIVE DNA-BINDING PROTEIN (CC_0111) FROM CAULOBACTER CRESCENTUS CB15 AT 1.62 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-01 |
| Detector | ADSC |
| Wavelength(s) | 1.0000, 0.9796, 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.891, 50.891, 121.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.940 - 1.620 |
| R-factor | 0.146 |
| Rwork | 0.145 |
| R-free | 0.16673 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.568 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.720 | 1.660 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 20981 | |
| <I/σ(I)> | 19.3 | 3.7 |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 7.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.8 | 277 | 1.0M sodium citrate, 0.1M Tris pH 7.0, 0.2M NaCl, pH 7.8, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
